Gemini EM Reader scans. 384 only) Selects either 96-well or 384-well plate for a stand-alone read. #ID5) HTRF Detection System (Molecular Devices cat. 20 attomol ATP per well. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. FlexStation 3 Multi Mode Microplate Reader. Plate formats. 2 with 1000 cells. 340 PC384. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. All stand-alone instrument functions can be controlled using the software. ,LTD (CAT#: STEM-S. QC tools and IQOQ services. The drawing shows a standard microplate of 15 mm height covered with a lid. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. ) 0. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. 6-well tissue-culture treated (TC) plate, sterile. These robust readers have been placed in labs from Antarctica to the. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. It features a modular design that allows users to add or change detection cartridges according to their needs. in every plate. Fluorescence Microplate Reader Comparison Chart. X. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. 4 Equipment. * Note: Programming the washer with a final aspirate height of 4. (or plate reader). The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. The plate reader can measure multiple readouts, including UV and. This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Figure 1. 96-384. 0 out of 5 $ 14,499. Molecular Devices Spectramax. . Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Ideal for miniaturized 384 well fluorescent. 5 mP 1536–2. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. and the A1 corner of the plate adapter (Figure 1-2). 314 low lamp level warning. Compare specifications and request a quote from leading suppliers. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. By measuring optical pathlength, it. x. A = Log 10 (I 0 /I). 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. or use the modified protocol below for the generation of NPCs (see Note 1). Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 384-well format. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. x. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. The equation that allows one to calculate absorbance from % transmittance is. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. 96 deep well plate: packed in sleeves of 6 , 24 per case. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. Efficiency. x. Microplates, cuvettes (via adapter) Detectors. SpectraMax iD5. 96-, 384-, and 1536-well microplates. Supplier: Molecular Devices. Important things to consider when purchasing a multimode microplate reader. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. for ELISAs and most cell-based assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Made from polypropylene with conical well profiles to. The assay methods covered include. ©2017 Molecular Devices, LLC . Dynamic range > 6 decades . Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. External Dimensions without lid (mm). com. 0. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 과 같은 Parameter를 검사하여 평가할 수 있습니다. within each well. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. VMax . It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. Absorbance cuvette port. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Both readers were heated to 37ºC prior to setting up the assay. The SpectraTest validation. 00. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Features. 616, 665 nm EM filters 0200-7011. Microplate format. Multimode Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. e. With optimized reagents, option to operate the. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. For more sample throughput, the system canThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. x. For nearly 30 years, Molecular Devices has provided scientists with . None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Automated live cell imaging – cell counting and confluence. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Next generation SpectraMax® absorbance microplate readers. 9. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. , San Diego, CA, USA) such that the. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 303 unable to cal dark current. x. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Expand the Microplates tree. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. 25mm: Plate width: 85. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. 96- and 384-well microplates. 2. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. 96/384-well plate formats. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Next-generation luminescence plate reader. Figure 1. molecular interaction studies, and nucleic acid quantification, as well. Molecular Devices products are warranted to meet the stated specifications. Bioz Stars score: 86/100, based on 9 PubMed citations. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Published Dec 16, 2020 | Updated Dec 16, 2020. #3-000-510)Corning® 384 well microplate, low volume poly-D-lysine coated surface, black polystyrene, round bottom, sterile, lid, pack of 10; Synonyms: 384 multiwell plates,384 well low volume plates,384 well microplates,384 well microtiter plates,384 well plates,384 well tissue culture treated plates; find -CLS3643 MSDS, related peer-reviewed papers,. Recertification service. That explains the lower values on the LmaxII384 plate reader. 12, 24, 96 & 384-well SBS microplates; Range of 96, 384-well SBS microplates . Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. x. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. x. 8 mm distance between well bottoms to plate bottom. Algorithms are optimized for accurate cell detection and. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. minutes and begin reading microplates with seven mouse clicks. In simplest terms, a plate reader measures and. #3-000-510)Page 154: Ordering Parts And Accessories. x. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. 2. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Other technologies such as imaging. 030 fmol Fluorescence Polarization (FP). x. A. The FLIPR 384 system comes complete with 384. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. 48 W x 12. Get Quote . com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. The small-footprint Multiwash+ Microplate Washer quickly washes with both 96 and 384-well plates, has 20 standard wash protocols, and a simple LCD screen interface to customize speed, volume, soak time, and aspiration settings. 더 많은. Following the flushing of the machine as described in Section 3. In addition, SoftMax Pro SoftwareSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. (96- and 384-well with pipetting) 6-384. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. #S1207. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. 96- and 384-well. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. 48 ± 0. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. were pipetted into a 96-well black-walled μclear plate. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. Two high-energy xenon flash lamps: Reading capabilities. 6-, 12-, 24-, 48-, 96-, and 384-well microplates; Extended research windows available; Temperature control; Returnable samples; Repeatable experiments;The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. ©2013 Molecular Devices, LLC • 6/13 • Printed in USA • PN: 0120-1523. Each kit is sufficient. ,LTD (CAT#: STEM-S. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. There is no product in the shopping cart, buy it!. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. x. –Min. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Service parts for these models are not being produced and are no longer in stock. A rinse program is included for quick and easy daily. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 0. x. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. Loading Buffer was also added to a set of cell-free wells for background controls. 5in (46. Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured Review. Industry standard disposable (choose from recommended list). After reading the ELISA plate on both readers, each standard curve was nearly identical. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. A 96- and 384-well automated washer. U-bottom: 30-40 µl. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. 7°C (gradient heating design). For more sample throughput, the system can6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Select Consumables. Source plate capacity; 1 x plates. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Key benefits • Assess cell confluence objectively and quantitatively. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. Catalog number: AB1384W. San Jose, Calif. Access this by going to. run on the FlexStation 3 reader in 384-well microplate with the FLIPR® Calcium 4 Assay Kit. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Kinetic assays were read every 20-28 seconds for one hour,measurements in compatible Molecular Devices® microplate readers. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. Uncontrolled Copy for Training and Reference Purposes Only . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Molecular Devices makes. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 1 pM 1536-well: 0. QuickSync. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Dimensions: 18. Inquiry Basket. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Summary of Contents for Molecular Devices SpectraMax M2. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. The analyzed results are. UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Download PDF. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Industry standard disposable (choose from recommended list). NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. All stand-alone instrument functions can be controlled using the software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 6-384. 3. 5 ng per well. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. If an organoid line forms small organoids in the 24-well semi. 7. Description. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. x. Instrument settings are listed in Table 1. Can read 96 well plates (optional 384 well). Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. $913. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. That explains the lower values on the LmaxII384 plate reader. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . 96-384. Includes PC and software. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. 96-well fluorescent cell dilution series, top-read. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. the Molecular Devices SPECTRAmax® 190 and 250 plate. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. #6590-0118) Filter 665/10 (Molecular Devices cat. a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. A control well was set up to verify. 1 fmol luciferase per well. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 0 mP 384–1. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 7°C and the bottom to 36. $245. 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 96-, 384-, and 1536. 96-Deep Round or Square Well Plates. For Raman excitation, a continuous-wave (CW) laser at 532 nm (Verdi V-18, Coherent) was used. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 3860 N First Street. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Temperature Control: Ambient +5C up to 42C. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. The opaque walls prevent stray light from being detected from neighbouring. x. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. x. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. An industry-renowned instrument for monitoring GPCRs and ion channels. Instructions for adding new plates: CSI/CP2: Add plate. #PLUS 384) UV-transparent 96-well microplates (Corning cat. #6590-0118) Filter 665/10 (Molecular Devices cat. For more sample throughput, the system canabsorbance plate readers. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Fluorescence Microplate Reader Comparison Chart. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. M2. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Assay setup for a 384-well low-volume plate. XML file. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Figure 1. Reading Capabilities: cuvette or microplate. For SpectraMax L or LMax II, LMax II 384 - no adapter used.